Journal article

A vanillic acid inducible expression system for Trypanosoma brucei

Abstract:: Reverse genetics in Trypanosoma brucei is dependent on the tetracycline inducible system for the precise control over the expression of both genes and dsRNA. Another independent inducible system for trypanosomes would enable the control of the activities of two different genes in the same cell, providing greater experimental sophistication. Here, I describe the development of the vanillic acid based inducible expression system for T. brucei, which operates independently of, and can be used in parallel with the tetracycline inducible system.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Sunter, J. (2016). A vanillic acid inducible expression system for Trypanosoma brucei. Molecular and Biochemical Parasitology.

MLA Style

Sunter, J. “A Vanillic Acid Inducible Expression System for Trypanosoma Brucei.” Molecular and Biochemical Parasitology, 2016.

Chicago Style

Sunter, J. 2016. “A Vanillic Acid Inducible Expression System for Trypanosoma Brucei.” Molecular and Biochemical Parasitology.
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Files:: 1-s2.0-S0166685116300299-main.pdf

(Preview, Version of record, pdf, 910.0KB, Terms of use)

Publisher copy:: 10.1016/j.molbiopara.2016.04.001

Authors

+ Sunter, J More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Pathology Dunn School
Role:: Author

+ Wellcome Trust More from this funder

Grant:: 104627/Z/14/Z; 108445/Z/15/Z

Publisher:: Elsevier
Journal:: Molecular and Biochemical Parasitology More from this journal
Publication date:: 2016-04-07
Acceptance date:: 2016-04-01
DOI:: 10.1016/j.molbiopara.2016.04.001
EISSN:: 1872-9428
ISSN:: 0166-6851

Keywords:: inducible expression

gene regulation

Trypanosoma brucei

vanillic acid
Pubs id:: pubs:614589
UUID:: uuid:0208293a-1adb-4479-9bf9-9931f3cf5f0f
Local pid:: pubs:614589
Source identifiers:: 614589
Deposit date:: 2016-04-08
ARK identifier:: ark:/29072/ora_0208293a1adb44799bf99931f3cf5f0f

Terms of use

Copyright holder:: J Sunter
Copyright date:: 2016
Notes:: © 2016 Published by Elsevier B.V. This is an open access article under the CC BY license.

Licence:: CC Attribution (CC BY)

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