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Journal article

Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding.

Abstract:

Electron cryotomography has unique potential for three-dimensional visualization of macromolecular complexes at work in their natural environment. This approach is based on reconstructing three-dimensional volumes from tilt series of electron micrographs of cells preserved in their native states by vitrification. Resolutions of 5-8 nm have already been achieved and the prospects for further improvement are good. Since many intracellular activities are conducted by complexes in the megadalton ...

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Publication status:
Published

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Journal:
Biophysical chemistry
Volume:
100
Issue:
1-3
Pages:
577-591
Publication date:
2003-01-01
DOI:
EISSN:
1873-4200
ISSN:
0301-4622
URN:
uuid:0202f54a-d537-48cf-9a6d-3c070915de8c
Source identifiers:
104029
Local pid:
pubs:104029

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