- Abstract:
-
Electron cryotomography has unique potential for three-dimensional visualization of macromolecular complexes at work in their natural environment. This approach is based on reconstructing three-dimensional volumes from tilt series of electron micrographs of cells preserved in their native states by vitrification. Resolutions of 5-8 nm have already been achieved and the prospects for further improvement are good. Since many intracellular activities are conducted by complexes in the megadalton ...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/s0301-4622(02)00307-1
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- Journal:
- Biophysical chemistry
- Volume:
- 100
- Issue:
- 1-3
- Pages:
- 577-591
- Publication date:
- 2003-01-01
- DOI:
- EISSN:
-
1873-4200
- ISSN:
-
0301-4622
- URN:
-
uuid:0202f54a-d537-48cf-9a6d-3c070915de8c
- Source identifiers:
-
104029
- Local pid:
- pubs:104029
- Language:
- English
- Keywords:
- Copyright date:
- 2003
Journal article
Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding.
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