Repo-Man/PP1 regulates heterochromatin formation in interphase.

Journal article

Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.

Authors: de Castro, I; Budzak, J; Di Giacinto, M; Ligammari, L; Gokhan, E

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Springer Nature
• Journal: Nature Communications
• Volume: 8
• Pages: 14048
• Publication date: 2017-01-16
• Acceptance date: 2016-11-23
• DOI: 10.1038/ncomms14048
• EISSN: 2041-1723
• ISSN: 2041-1723
• Language: English
• Keywords: Cell division; Epigenetics; Chromatin