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Insect transformation with piggyBac: getting the number of injections just right

Abstract:

The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilised for insect germline transformation. The described efficiency of this process is variable in published literature, and a comprehensive review of transformation efficiency in insects is lacking. This study compares and contrasts a...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1111/imb.12220

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Zoology
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Zoology
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Zoology
Morrison, NI More by this author
Publisher:
Royal Entomological Society Publisher's website
Journal:
Insect Molecular Biology Journal website
Volume:
25
Issue:
3
Pages:
259–271
Publication date:
2016-03-30
DOI:
EISSN:
1365-2583
ISSN:
0962-1075
URN:
uuid:0150b153-0fbf-4e0a-861b-605fa5ed1bba
Source identifiers:
581106
Local pid:
pubs:581106

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