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A simple low-cost device enables four epi-illumination techniques on standard light microscopes

Abstract:

Back-scattering darkfield (BSDF), epi-fluorescence (EF), interference reflection contrast (IRC), and darkfield surface reflection (DFSR) are advanced but expensive light microscopy techniques with limited availability. Here we show a simple optical design that combines these four techniques in a simple low-cost miniature epi-illuminator, which inserts into the differential interference-contrast (DIC) slider bay of a commercial microscope, without further additions required. We demonstrate wit...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/srep20729

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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Condensed Matter Physics
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Publisher:
Nature Publishing Group Publisher's website
Journal:
Scientific Reports Journal website
Volume:
6
Pages:
Article: 20729
Publication date:
2016-02-08
DOI:
ISSN:
2045-2322
URN:
uuid:013b2ee6-3f6d-4735-8c80-d8c718c5919c
Source identifiers:
599546
Local pid:
pubs:599546

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