- Abstract:
-
There is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific for the 16S ribosomal RNA gene (rrs) of pathogenic and intermediate group Leptospira species. The lower limit of detection was 10 genomic equivalents/reaction, and analytical specificity was high; we observed positive reactions for pathogenic/intermediate groups and negative reactions for non-pathogenic Leptospira species and other bacter...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.4269/ajtmh.2011.10-0473
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- Journal:
- The American journal of tropical medicine and hygiene
- Volume:
- 84
- Issue:
- 4
- Pages:
- 614-620
- Publication date:
- 2011-04-05
- DOI:
- EISSN:
-
1476-1645
- ISSN:
-
0002-9637
- URN:
-
uuid:00df7da9-abdd-48ec-bd06-0587c55328a8
- Source identifiers:
-
131161
- Local pid:
- pubs:131161
- Copyright date:
- 2011
Journal article
Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand.
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