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Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis.

Abstract:
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

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Publisher copy:
10.3402/jev.v2i0.19671

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Women's and Reproductive Health
Role:
Author


Journal:
Journal of extracellular vesicles More from this journal
Volume:
2
Issue:
1
Publication date:
2013-01-01
DOI:
EISSN:
2001-3078


Language:
English
Keywords:
Pubs id:
pubs:421143
UUID:
uuid:00b76986-d477-47d3-85f8-b87b9e969827
Local pid:
pubs:421143
Source identifiers:
421143
Deposit date:
2014-12-23

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