DNA delivery systems based on complexes of DNA with synthetic polycations and their copolymers

Journal article

Block and graft copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) with 2-(trimethylammonio)ethyl methacrylate were synthesised and used for preparation of polyelectrolyte complexes with calf thymus DNA intended for targeted delivery of genes in vivo. In this study the effects of the speed of component mixing, total concentration of polymers, ionic strength of solvents, copolymer structure and content of HPMA in the copolymers on parameters of the polyelec...

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Authors: Oupický, D; Koňák, Č; Ulbrich, K; Wolfert, M; Seymour, L

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Elsevier
• Journal: Journal of Controlled Release
• Volume: 65
• Issue: 1-2
• Pages: 149-171
• Publication date: 2000-02-25
• Acceptance date: 1999-07-07
• DOI: 10.1016/S0168-3659(99)00249-7
• EISSN: 1873-4995
• ISSN: 0168-3659
• Keywords: Light scattering methods; Transfection efficiency; Calf thymus DNA; Polycations; Stability of complexes; SBTMR; Self-assembly; Interpolyelectrolyte complexes; Diblock and graft copolymers