N/O-methylation of DNA can be cytotoxic and mutagenic; therefore, enzymes that reverse DNA methylation are essential for organism survival. Several 2-oxoglutarate-dependent oxygenases and methyltransferases that remove a methyl group from a methylated DNA base have been identified. Studies of their kinetics and search for their inhibitors have been retarded by the lack of an approach to directly quantitate DNA substrates and products that differ by a single methyl group. Here, we introduce su...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Direct analysis of enzyme-catalyzed DNA demethylation.
If you are the owner of this record, you can report an update to it here: Report update to this record