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Direct analysis of enzyme-catalyzed DNA demethylation.

Abstract:

N/O-methylation of DNA can be cytotoxic and mutagenic; therefore, enzymes that reverse DNA methylation are essential for organism survival. Several 2-oxoglutarate-dependent oxygenases and methyltransferases that remove a methyl group from a methylated DNA base have been identified. Studies of their kinetics and search for their inhibitors have been retarded by the lack of an approach to directly quantitate DNA substrates and products that differ by a single methyl group. Here, we introduce su...

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Publication status:
Published

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Publisher copy:
10.1021/ac9010556

Authors


Karkhanina, AA More by this author
Mecinović, J More by this author
Musheev, MU More by this author
Krylova, SM More by this author
Petrov, AP More by this author
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Journal:
Analytical chemistry
Volume:
81
Issue:
14
Pages:
5871-5875
Publication date:
2009-07-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:00591c79-e82c-4304-8eca-85a51d49639c
Source identifiers:
41280
Local pid:
pubs:41280

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