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Journal article

Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA.

Abstract:

We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif, 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) has very high affinity for AT relative to all other Watson-Crick base pairs in DNA. Complexes containing two or more substitutions with this nucleotide analogue are stable at pH 7.0, even though they contain several C.GC base triplets. These modified triplex-fo...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gkh776

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Journal:
Nucleic acids research
Volume:
32
Issue:
15
Pages:
4439-4447
Publication date:
2004-01-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:0033337c-2777-47bc-8c84-2401bd53ccd7
Source identifiers:
400050
Local pid:
pubs:400050

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