Journal article
Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA.
- Abstract:
-
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif, 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) has very high affinity for AT relative to all other Watson-Crick base pairs in DNA. Complexes containing two or more substitutions with this nucleotide analogue are stable at pH 7.0, even though they contain several C.GC base triplets. These modified triplex-fo...
Expand abstract
- Publication status:
- Published
Actions
Authors
Bibliographic Details
- Journal:
- Nucleic acids research
- Volume:
- 32
- Issue:
- 15
- Pages:
- 4439-4447
- Publication date:
- 2004-01-01
- DOI:
- EISSN:
-
1362-4962
- ISSN:
-
0305-1048
- Source identifiers:
-
400050
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:400050
- UUID:
-
uuid:0033337c-2777-47bc-8c84-2401bd53ccd7
- Local pid:
- pubs:400050
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2004
If you are the owner of this record, you can report an update to it here: Report update to this record