Calcium increases endocytotic vesicle size and accelerates membrane fission in insulin-secreting INS-1 cells.

Journal article

In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of ...

Expand abstract

Authors: MacDonald, P; Eliasson, L; Rorsman, P

• Publication status: Published
• Journal: Journal of cell science
• Volume: 118
• Issue: Pt 24
• Pages: 5911-5920
• Publication date: 2005-12-01
• DOI: 10.1242/jcs.02685
• EISSN: 1477-9137
• ISSN: 0021-9533
• Language: English
• Keywords: Nitriles; Patch-Clamp Techniques; Insecticides; Membrane Potentials; Pyrethrins; Humans; Membrane Fusion; Calcium Channels; Glucose; Sweetening Agents; Insulin-Secreting Cells; Animals; Cell Line; Calcineurin; Dose-Response Relationship, Drug; Transport Vesicles; Calcium