Impaired phagocytic mechanism in annexin 1 null macrophages.

Journal article

The role of the anti-inflammatory protein annexin-A1 (Anx-A1) in the phagocytic process has been investigated using a murine bone marrow culture-derived macrophage model from Anx-A1(+/+) and Anx-A1(-/-) mice. Macrophages prepared from Anx-A1(-/-) mice exhibited a reduced ingestion of zymosan, Neisseria meningitidis or sheep red blood cells, when compared to Anx-A1(+/+) cells and in the case of zymosan this effect was also mirrored by a reduced clearance in vivo when particles were injected into the peritoneal cavity of Anx-A1(-/-) mice. The ablation of the Anx-A1 gene did not cause any apparent cytoskeletal defects associated with particle ingestion but the cell surface expression of the key adhesion molecule CD11b was depressed in the Anx-A1(-/-) cells providing a possible explanation for the attenuated phagocytic potential of these cells. The production of the cytokines TNFalpha and IL-6 was increased in Anx-A1(-/-) macrophages following phagocytosis of all types of particle.

Authors: Yona, S; Heinsbroek, SE; Peiser, L; Gordon, S; Perretti, M

• Publication status: Published
• Journal: British journal of pharmacology
• Volume: 148
• Issue: 4
• Pages: 469-477
• Publication date: 2006-06-01
• DOI: 10.1038/sj.bjp.0706730
• EISSN: 1476-5381
• ISSN: 0007-1188
• Language: English
• Keywords: Phagocytosis; Annexin A1; Antigens, CD11b; Zymosan; Animals; Mice; Flow Cytometry; Macrophages; Interleukin-6; Tumor Necrosis Factor-alpha