Protein production and purification. - ORA - Oxford University Research Archive

Abstract:: In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

Publication status:: Published

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APA Style

Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, B., Bray, J., Gileadi, O., Knapp, S., Oppermann, U., Arrowsmith, C., Hui, R., Ming, J., dhe-Paganon, S., Park, H., Savchenko, A., Yee, A., Edwards, A., Vincentelli, R., Cambillau, C., Kim, R., … Hung, L. (2008). Protein production and purification. Nature Methods, 5(2), 135–146.

MLA Style

Gräslund, S, et al. “Protein Production and Purification.” Nature Methods, vol. 5, no. 2, 2008, pp. 135–46.

Chicago Style

Gräslund, S, P Nordlund, J Weigelt, et al. 2008. “Protein Production and Purification.” Nature Methods 5 (2): 135–46.
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Publisher copy:: 10.1038/nmeth.f.202

Authors

+ Gräslund, S More by this author

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+ Nordlund, P More by this author

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+ Weigelt, J More by this author

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+ Hallberg, B More by this author

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+ Bray, J More by this author

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Journal:: Nature methods More from this journal
Volume:: 5
Issue:: 2
Pages:: 135-146
Publication date:: 2008-02-01
DOI:: 10.1038/nmeth.f.202
EISSN:: 1548-7105
ISSN:: 1548-7091

Language:: English
Keywords:: Northeast Structural Genomics Consortium

Chemistry, Physical

Recombinant Proteins

Protein Engineering

Chemical Fractionation

China Structural Genomics Consortium

Proteomics

Structural Genomics Consortium
Pubs id:: pubs:29159
UUID:: uuid:88c53547-b930-4a16-a155-4a9f77c2ab3b
Local pid:: pubs:29159
Source identifiers:: 29159
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_88c53547b9304a16a1554a9f77c2ab3b

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Copyright date:: 2008

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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