Journal article
Quantitative mass imaging of single biological macromolecules
- Abstract:
- The cellular processes underpinning life are orchestrated by proteins and their interactions. The associated structural and dynamic heterogeneity, despite being key to function, poses a fundamental challenge to existing analytical and structural methodologies. We used interferometric scattering microscopy to quantify the mass of single biomolecules in solution with 2% sequence mass accuracy, up to 19-kilodalton resolution, and 1-kilodalton precision. We resolved oligomeric distributions at high dynamic range, detected small-molecule binding, and mass-imaged proteins with associated lipids and sugars. These capabilities enabled us to characterize the molecular dynamics of processes as diverse as glycoprotein cross-linking, amyloidogenic protein aggregation, and actin polymerization. Interferometric scattering mass spectrometry allows spatiotemporally resolved measurement of a broad range of biomolecular interactions, one molecule at a time.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 2.2MB, Terms of use)
-
- Publisher copy:
- 10.1126/science.aar5839
Authors
+ European Research Council
More from this funder
- Funding agency for:
- Kukura, P
- Grant:
- Starting Investigator Grant (Nanoscope, 337577)
+ Engineering and Physical Sciences Research Council
More from this funder
- Funding agency for:
- Tkachenko, O
- Benesch, JLP
- Grant:
- Studentship
- EP/J01835X/1
+ St John’s College, Oxford
More from this funder
- Funding agency for:
- Tkachenko, O
- Grant:
- Studentship
+ Waters Corp
More from this funder
- Funding agency for:
- Chandler, SA
- Benesch, JLP
- Grant:
- BB/L017067/1
- EP/J01835X/1
+ Biotechnology and Biological Sciences Research
Council
More from this funder
- Funding agency for:
- Chandler, SA
- Benesch, JLP
- Grant:
- BB/L017067/1
- EP/J01835X/1
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science More from this journal
- Volume:
- 360
- Issue:
- 6387
- Pages:
- 423-427
- Publication date:
- 2018-04-27
- Acceptance date:
- 2018-03-26
- DOI:
- EISSN:
-
1095-9203
- ISSN:
-
0036-8075
- Pubs id:
-
pubs:843982
- UUID:
-
uuid:b6403cfa-56ae-4675-981b-cc77f69e7dc6
- Local pid:
-
pubs:843982
- Source identifiers:
-
843982
- Deposit date:
-
2018-04-24
Terms of use
- Copyright holder:
- Young et al
- Copyright date:
- 2018
- Notes:
- Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at: https://doi.org/10.1126/science.aar5839
If you are the owner of this record, you can report an update to it here: Report update to this record