Journal article
Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions
- Abstract:
- Oligomeric proteins assemble with exceptional selectivity, even in the presence of closely related proteins, to perform their cellular roles. We show that most proteins related by gene duplication of an oligomeric ancestor have evolved to avoid hetero-oligomerization and that this correlates with their acquisition of distinct functions. We report how coassembly is avoided by two oligomeric small heat-shock protein paralogs. A hierarchy of assembly, involving intermediates that are populated only fleetingly at equilibrium, ensures selective oligomerization. Conformational flexibility at noninterfacial regions in the monomers prevents coassembly, allowing interfaces to remain largely conserved. Homomeric oligomers must overcome the entropic benefit of coassembly and, accordingly, homomeric paralogs comprise fewer subunits than homomers that have no paralogs.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
-
(Preview, Accepted manuscript, pdf, 19.8MB, Terms of use)
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- Publisher copy:
- 10.1126/science.aam7229
Authors
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science More from this journal
- Volume:
- 359
- Issue:
- 6378
- Pages:
- 930-935
- Publication date:
- 2018-02-23
- Acceptance date:
- 2018-01-08
- DOI:
- EISSN:
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1095-9203
- ISSN:
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0036-8075
- Language:
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English
- Pubs id:
-
pubs:827320
- UUID:
-
uuid:93121a0f-46ba-4070-b6d3-05514d82363f
- Local pid:
-
pubs:827320
- Source identifiers:
-
827320
- Deposit date:
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2018-03-03
Terms of use
- Copyright holder:
- Hochberg et al
- Copyright date:
- 2018
- Notes:
- Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at: https://doi.org/10.1126/science.aam7229
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