Metabolic inhibition and low internal ATP activate K-ATP channels in rat dopaminergic substantia nigra neurones.

Journal article

The patch-clamp technique was used to study whole-cell currents of acutely dissociated rat substantia nigra (SN) neurones. In perforated-patch current-clamp recordings, inhibition of mitochondrial metabolism by rotenone (5 microM) produced a hyperpolarisation and inhibited electrical activity. These effects were reversed by the sulphonylureas tolbutamide (0.5 mM) or glibenclamide (0.5 microM). Under voltage-clamp conditions, rotenone induced a time- and voltage-independent K+ current which wa...

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Authors: Röper, J; Ashcroft, F

• Publication status: Published
• Journal: Pflugers Archiv : European journal of physiology
• Volume: 430
• Issue: 1
• Pages: 44-54
• Publication date: 1995-05-01
• DOI: 10.1007/bf00373838
• EISSN: 1432-2013
• ISSN: 0031-6768
• Language: English
• Keywords: Animals; Potassium Channel Blockers; Glyburide; Sulfonylurea Compounds; Potassium; Biological Transport; Action Potentials; Patch-Clamp Techniques; Rats, Inbred Strains; Rotenone; Mitochondria; Catecholamines; Substantia Nigra; Male; Adenosine Triphosphate; Potassium Channels; Rats; Cell Separation; Neurons