Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters.

Journal article

We demonstrate that multiple SP1 protein:DNA binding sites confer enhancer-independent activation on the HIV-1 and globin gene promoters. This activation process can be achieved either by DNA replication of the promoter-containing plasmid or by high concentrations of input plasmid DNA used in the transfections. In the case of HIV-1, the three SP1 sites adjacent to the promoters TATA box are essential for this activation process. Furthermore, the human beta globin gene, which is normally depen...

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Authors: Proudfoot, N; Lee, B; Monks, J

• Publication status: Published
• Journal: New biologist
• Volume: 4
• Issue: 4
• Pages: 369-381
• Publication date: 1992-04-01
• ISSN: 1043-4674
• Language: English
• Keywords: TATA Box; DNA; Globins; Base Sequence; Molecular Sequence Data; Humans; Enhancer Elements, Genetic; Sp1 Transcription Factor; Promoter Regions, Genetic; DNA Replication; Binding Sites; Transcription, Genetic; Plasmids; Hela Cells; HIV-1; Genes, Viral