Engineering a camelid antibody fragment that binds to the active site of human lysozyme and inhibits its conversion into amyloid fibrils.

Journal article

A single-domain fragment, cAb-HuL22, of a camelid heavy-chain antibody specific for the active site of human lysozyme has been generated, and its effects on the properties of the I56T and D67H amyloidogenic variants of human lysozyme, which are associated with a form of systemic amyloidosis, have been investigated by a wide range of biophysical techniques. Pulse-labeling hydrogen-deuterium exchange experiments monitored by mass spectrometry reveal that binding of the antibody fragment strongl...

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Authors: Chan, P; Pardon, E; Menzer, L; De Genst, E; Kumita, JR

• Publication status: Published
• Journal: Biochemistry
• Volume: 47
• Issue: 42
• Pages: 11041-11054
• Publication date: 2008-10-01
• DOI: 10.1021/bi8005797
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Recombinant Proteins; Animals; Catalytic Domain; Nuclear Magnetic Resonance, Biomolecular; Immunoglobulin Fragments; Amino Acid Sequence; Molecular Sequence Data; Sequence Homology, Amino Acid; Muramidase; Camels; Antibody Affinity; Humans; Protein Engineering; Amyloid