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Phase-based segmentation of cells from brightfield microscopy

Abstract:

Segmentation of transparent cells in brightfield microscopy images could facilitate the quantitative analysis of corresponding fluorescence images. However, this presents a challenge due to irregular morphology and weak intensity variation, particularly in ultra-thin regions. A boundary detection technique is applied to a series of variable focus images whereby a level set contour is initialised on a defocused image with improved intensity contrast, and subsequently evolved towards the correc...

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Publication status:
Published

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Publisher copy:
10.1109/ISBI.2007.356787

Authors


Pages:
57-60
Host title:
2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3
Publication date:
2007-01-01
DOI:
ISSN:
1945-7928
Source identifiers:
65053
ISBN:
9781424406715
Keywords:
Pubs id:
pubs:65053
UUID:
uuid:ffaec014-81ff-4aa2-9cd6-73eb54c7cc05
Local pid:
pubs:65053
Deposit date:
2012-12-19

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