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Low-input capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells

Abstract:
Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.21769/BioProtoc.2645

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
Weatherall Inst of Molecular Medicine
Oxford college:
University College
Role:
Author
ORCID:
0000-0002-4016-6158
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
RDM Clinical Laboratory Sciences
Role:
Author
ORCID:
0000-0002-5034-0869
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
RDM Clinical Laboratory Sciences
Role:
Author
ORCID:
0000-0002-4108-4357
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
RDM Clinical Laboratory Sciences
Role:
Author
ORCID:
0000-0002-8955-7256
Publisher:
Bio-Protocol Publisher's website
Journal:
Bio-Protocol Journal website
Volume:
7
Issue:
23
Publication date:
2017-12-05
DOI:
EISSN:
2331-8325
Pubs id:
pubs:813008
URN:
uri:ffa48ab8-905f-4832-8563-b3667680c372
UUID:
uuid:ffa48ab8-905f-4832-8563-b3667680c372
Local pid:
pubs:813008

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