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Separation of human serum albumin and human immunoglobulins using carrier phase ultrafiltration.

Abstract:

The fractionation of the plasma proteins human serum albumin (HSA) and human immunoglobulins (HIgG) using the combination of two newly developed techniques, pulsed sample injection technique and carrier phase ultrafiltration (CPUF), is discussed in this paper. The effects of pH and ionic strength on the transmission of a single protein (i.e., either HSA or HIgG) through 100 and 300 kDa MWCO polyethersulfone (PES) membranes were quantified using the pulsed sample injection technique. The exper...

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Publication status:
Published

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Publisher copy:
10.1021/bp0343716

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Biotechnology progress
Volume:
20
Issue:
4
Pages:
1103-1112
Publication date:
2004-01-01
DOI:
EISSN:
1520-6033
ISSN:
8756-7938
URN:
uuid:ff56fa71-708a-4a26-aba7-8dc1794f55fa
Source identifiers:
61923
Local pid:
pubs:61923

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