Intrathymic delivery of plasmid-encoding endoplasmic reticulum signal-sequence-deleted MHC class I alloantigen can induce long-term allograft survival.

Journal article

Intrathymic (IT) delivery of donor alloantigen is a potent strategy to induce operational tolerance. In this study we determined whether this effect was dependent on direct allorecognition of the tolerogen. Ten microgrammes of plasmid, encoding either the wildtype major histocompatibility complex (MHC) class I molecule K(b) or a truncated form in which the signal sequence for translocation into the endoplasmic reticulum was deleted, preventing cell surface expression and direct allorecognitio...

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Authors: Spriewald, B; Ensminger, S; Jenkins, S; Morris, P; Wood, K

• Publication status: Published
• Journal: Transplant international : official journal of the European Society for Organ Transplantation
• Volume: 17
• Issue: 8
• Pages: 458-462
• Publication date: 2004-09-01
• DOI: 10.1007/s00147-004-0741-4
• EISSN: 1432-2277
• ISSN: 0934-0874
• Language: English
• Keywords: Mice, Inbred CBA; Plasmids; Mice, Inbred Strains; Endoplasmic Reticulum; Graft Survival; Transplantation, Homologous; Heart Transplantation; Mice, Inbred C57BL; Mice; Major Histocompatibility Complex; Animals; Histocompatibility Testing; Cloning, Molecular; Histocompatibility Antigens Class I