Microtubular organization visualized by immunofluorescence microscopy during erythrocytic schizogony in Plasmodium falciparum and investigation of post-translational modifications of parasite tubulin.

Journal article

We describe a novel procedure for the immunofluorescent investigation of Plasmodium falciparum. This has allowed us to visualize clearly microtubular structures and their changing conformation through the erythrocytic cell-cycle, to the stage of cytodifferentiation leading to merozoite release. The images of spindle development we observed, together with an analysis of nuclear body numbers in large numbers of parasites, indicate that there is an apparent asynchrony in chromosomal multiplicati...

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Authors: Read, M; Sherwin, T; Holloway, S; Gull, K; Hyde, J

• Publication status: Published
• Journal: Parasitology
• Volume: 106 ( Pt 3)
• Issue: 03
• Pages: 223-232
• Publication date: 1993-04-01
• DOI: 10.1017/s0031182000075041
• EISSN: 1469-8161
• ISSN: 0031-1820
• Language: English
• Keywords: Microtubules; Cell Division; Tubulin; Fluorescent Antibody Technique; Time Factors; Antibodies, Monoclonal; Protozoan Proteins; Erythrocytes; Plasmodium falciparum; Animals; Humans; Cell Differentiation; Protein Processing, Post-Translational