Characterisation of a mobile protein-binding epitope in the translocation domain of colicin E9.

Journal article

The 61 kDa colicin E9 protein toxin enters the cytoplasm of susceptible cells by interacting with outer membrane and periplasmic helper proteins, and kills them by hydrolysing their DNA. The membrane translocation function is located in the N-terminal domain of the colicin, with a key signal sequence being a pentapeptide region that governs the interaction with the helper protein TolB (the TolB box). Previous NMR studies (Collins et al., 2002 J. Mol. Biol. 318, 787-804) have shown that the N-terminal 83 residues of colicin E9, which includes the TolB box, is largely unstructured and highly flexible. In order to further define the properties of this region we have studied a fusion protein containing residues 1-61 of colicin E9 connected to the N-terminus of the E9 DNase by an eight-residue linking sequence. 53 of the expected 58 backbone NH resonances for the first 61 residues and all of the expected 7 backbone NH resonances of the linking sequence were assigned with 3D (1)H-(13)C-(15)N NMR experiments, and the backbone dynamics of these regions investigated through measurement of (1)H-(15)N relaxation properties. Reduced spectral density mapping, extended Lipari-Szabo modelling, and fitting backbone R(2) relaxation rates to a polymer dynamics model identifies three clusters of interacting residues, each containing a tryptophan. Each of these clusters is perturbed by TolB binding to the intact colicin, showing that the significant region for TolB binding extends beyond the recognized five amino acids of the TolB box and demonstrating that the binding epitope for TolB involves a considerable degree of order within an otherwise disordered and flexible domain. Abbreviations : Im9, the immunity protein for colicin E9; E9 DNase, the endonuclease domain of colicin E9; HSQC, heteronuclear single quantum coherence; ppm, parts per million; DSS, 2,2-(dimethylsilyl)propanesulfonic acid; TSP, sodium 3-trimethylsilypropionate; T(1 - 61)-DNase fusion protein, residues 1-61 of colicin E9 connected to the N-terminus of the E9 DNase by an eight residue thrombin cleavage sequence.

Authors: Macdonald, C; Tozawa, K; Collins, E; Penfold, C; James, R

• Journal: Journal of biomolecular NMR
• Volume: 30
• Issue: 1
• Pages: 81-96
• Publication date: 2004-09-01
• DOI: 10.1023/b:jnmr.0000042963.71790.19
• EISSN: 1573-5001
• ISSN: 0925-2738
• Language: English
• Keywords: Molecular Weight; Amino Acid Sequence; Epitopes; Escherichia coli Proteins; Colicins; Binding Sites; Protein Conformation; Protein Binding; Deoxyribonucleases; Protein Structure, Tertiary; Biological Transport, Active; Nuclear Magnetic Resonance, Biomolecular; Carbon Isotopes; Quantum Theory; Escherichia coli; Nitrogen Isotopes