- Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
- Publication status:
- Peer review status:
- Peer reviewed
- Accepted Manuscript
- Copyright holder:
- Nature America, Inc.
- Copyright date:
- Copyright © 2013 Nature America, Inc. Citation: Howden, A. J. M., Geoghegan, V., Katsch, K., Efstathiou, G., Bhushan, B., Boutureira, O., … Acuto, O. (2013, March 10). QuaNCAT: quantitating proteome dynamics in primary cells. Nature Methods. Nature Publishing Group. http://doi.org/10.1038/nmeth.2401
QuaNCAT: quantitating proteome dynamics in primary cells.
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