- Abstract:
- Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Accepted Manuscript
- Files:
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(pdf, 1.7MB)
-
- Publisher copy:
- 10.1038/nmeth.2401
-
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- Publisher:
- Nature Publishing Group Publisher's website
- Journal:
- Nature Methods Journal website
- Volume:
- 10
- Issue:
- 4
- Pages:
- 343-346
- Publication date:
- 2013-03-05
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- URN:
-
uuid:fe61070d-d72b-4696-88d8-5b9adfb7efed
- Source identifiers:
-
383054
- Local pid:
- pubs:383054
- Copyright holder:
- Nature America, Inc.
- Copyright date:
- 2013
- Notes:
- Copyright © 2013 Nature America, Inc. Citation: Howden, A. J. M., Geoghegan, V., Katsch, K., Efstathiou, G., Bhushan, B., Boutureira, O., … Acuto, O. (2013, March 10). QuaNCAT: quantitating proteome dynamics in primary cells. Nature Methods. Nature Publishing Group. http://doi.org/10.1038/nmeth.2401
Journal article
QuaNCAT: quantitating proteome dynamics in primary cells.
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