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QuaNCAT: quantitating proteome dynamics in primary cells.

Abstract:
Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1038/nmeth.2401

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Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Methods Journal website
Volume:
10
Issue:
4
Pages:
343-346
Publication date:
2013-03-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:fe61070d-d72b-4696-88d8-5b9adfb7efed
Source identifiers:
383054
Local pid:
pubs:383054

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