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Sequence tag scanning: a new explorative strategy for recognition of unexpected protein alterations by nanoelectrospray ionization-tandem mass spectrometry.

Abstract:

Protein analysis by database search engines using tandem mass spectra is limited by the presence of unexpected protein modifications, sequence isoforms which may not be in the protein databases, and poor quality tandem mass spectrometry (MS/MS) of low abundance proteins. The analysis of expected protein modifications can be efficiently addressed by precursor ion scanning. However, it is limited to modifications that show such a characteristic loss in a peptide independent manner. We observed ...

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Publication status:
Published

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Publisher copy:
10.1002/pmic.200401152

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Volume:
5
Issue:
3
Pages:
667-674
Publication date:
2005-02-05
DOI:
EISSN:
1615-9861
ISSN:
1615-9853
URN:
uuid:fe41a804-5b56-4863-83c6-7d6c1cc79e96
Source identifiers:
22972
Local pid:
pubs:22972

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