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Journal article

Rare event detection using error-corrected DNA and RNA sequencing

Abstract:

Conventional next-generation sequencing techniques (NGS) have allowed for immense genomic characterization for over a decade. Specifically, NGS has been used to analyze the spectrum of clonal mutations in malignancy. Though far more efficient than traditional Sanger methods, NGS struggles with identifying rare clonal and subclonal mutations due to its high error rate of ~0.5–2.0%. Thus, standard NGS has a limit of detection for mutations that are >0.02 variant allele fraction (VAF). While ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3791/57509

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Role:
Author
Publisher:
MYJoVE Corporation
Journal:
Journal of Visualized Experiments More from this journal
Volume:
138
Issue:
2018
Article number:
e57509
Publication date:
2019-03-08
DOI:
ISSN:
1940-087X
Language:
English
Keywords:
Pubs id:
pubs:993824
UUID:
uuid:fdbc139c-5b6f-4e5b-a5f8-e99dc789475f
Local pid:
pubs:993824
Source identifiers:
993824
Deposit date:
2019-04-24

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