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UHRF1 Is a sensor for DNA interstrand crosslinks and recruits FANCD2 to initiate the Fanconi Anemia pathway

Abstract:

The Fanconi anemia (FA) pathway is critical for the cellular response to toxic DNA interstrand crosslinks (ICLs). Using a biochemical purification strategy, we identified UHRF1 as a protein that specifically interacts with ICLs invitro and invivo. Reduction of cellular levels of UHRF1 by RNAi attenuates the FA pathway and sensitizes cells to mitomycin C. Knockdown cells display a drastic reduction in FANCD2 foci formation. Using live-cell imaging, we observe that UHRF1 is rapidly recruited to...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1016/j.celrep.2015.02.053

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
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Funding agency for:
Liang, CC
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Funding agency for:
Liang, CC
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Funding agency for:
Zhan, B
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Funding agency for:
Cohn, M
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Publisher:
Cell Press Publisher's website
Journal:
Cell Reports Journal website
Volume:
10
Issue:
12
Pages:
1947-1957
Publication date:
2015-03-19
DOI:
EISSN:
2211-1247
URN:
uuid:fdbb4c49-f620-4187-a480-ba9ea48adb6b
Source identifiers:
517850
Local pid:
pubs:517850

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