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Structural and dynamical properties of a denatured protein. Heteronuclear 3D NMR experiments and theoretical simulations of lysozyme in 8 M urea.

Abstract:

Oxidized and reduced hen lysozyme denatured in 8 M urea at low pH have been studied in detail by NMR methods. 15N correlated NOESY and TOCSY experiments have provided near complete sequential assignment for both 1H and 15N resonances. Over 900 NOEs, including 130 (i, i + 2) and 23 (i, i + 3) NOEs, could be identified by analysis of the NOESY spectra of the denatured states, and 3J(HN, Halpha) coupling constants and 15N relaxation rates have been measured. The coupling constant and NOE data we...

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Publication status:
Published

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Publisher copy:
10.1021/bi970049q

Authors


Schwalbe, H More by this author
Fiebig, KM More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Atomic and Laser Physics
Grimshaw, SB More by this author
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Journal:
Biochemistry
Volume:
36
Issue:
29
Pages:
8977-8991
Publication date:
1997-07-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:fd94687b-0eb4-4bb5-b8d1-b2cc7ef8ddcf
Source identifiers:
15975
Local pid:
pubs:15975

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