Journal article
Structural and dynamical properties of a denatured protein. Heteronuclear 3D NMR experiments and theoretical simulations of lysozyme in 8 M urea.
- Abstract:
-
Oxidized and reduced hen lysozyme denatured in 8 M urea at low pH have been studied in detail by NMR methods. 15N correlated NOESY and TOCSY experiments have provided near complete sequential assignment for both 1H and 15N resonances. Over 900 NOEs, including 130 (i, i + 2) and 23 (i, i + 3) NOEs, could be identified by analysis of the NOESY spectra of the denatured states, and 3J(HN, Halpha) coupling constants and 15N relaxation rates have been measured. The coupling constant and NOE data we...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Biochemistry
- Volume:
- 36
- Issue:
- 29
- Pages:
- 8977-8991
- Publication date:
- 1997-07-01
- DOI:
- EISSN:
-
1520-4995
- ISSN:
-
0006-2960
- Source identifiers:
-
15975
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:15975
- UUID:
-
uuid:fd94687b-0eb4-4bb5-b8d1-b2cc7ef8ddcf
- Local pid:
- pubs:15975
- Deposit date:
- 2012-12-19
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- Copyright date:
- 1997
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