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Multisite phosphorylation of the 80 kDa (MARCKS) protein kinase C substrate in C3H/10T1/2 fibroblasts. Quantitative analysis of individual sites by solid-phase microsequencing.

Abstract:

A synthetic peptide, KKKKRFSFKKSFKLSGFSFKK, containing the phosphorylation sites of the acidic 80-87 kDa protein kinase C substrate was used to identify phosphopeptides in enzyme digests of this protein from mouse fibroblast C3H/10T1/2 cells. Stimulation of phosphorylation occurred, in vivo, with TPA at Ser7, Ser11 and Ser18, and, with two less potent phorbol esters, at Ser7 and Ser18. Okadaic acid effected a net phosphorylation of Ser7 and/or Ser11. Solid-phase sequencing showed that, in vit...

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Journal:
FEBS letters More from this journal
Volume:
297
Issue:
3
Pages:
285-291
Publication date:
1992-02-01
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
Language:
English
Keywords:
Pubs id:
pubs:487349
UUID:
uuid:fd00a783-ff39-4ea1-bf0c-02cd138e6b8b
Local pid:
pubs:487349
Source identifiers:
487349
Deposit date:
2014-10-24

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