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Comparison of overlapping peptide sets for detection of antiviral CD8 and CD4 T cell responses.

Abstract:

Increasing efforts are directed towards the development of effective vaccines through induction of virus-specific T cell responses. Although emerging data indicate a significant role of these cells in determining viral set point in infections such as HIV, there is as yet no consensus as to the best methods for assaying the breadth of these responses. In this study, we used sensitive interferon gamma-based intracellular cytokine staining (ICS) and Elispot assays to determine the optimal overla...

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Journal:
Journal of immunological methods More from this journal
Volume:
275
Issue:
1-2
Pages:
19-29
Publication date:
2003-04-01
DOI:
EISSN:
1872-7905
ISSN:
0022-1759

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