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In vitro reconstitution of the human RISC-loading complex.

Abstract:

Targeted gene silencing by RNAi requires the RNA-induced silencing complex (RISC), whose core component is the protein Argonaute (Ago) bound to a microRNA (miRNA) or an siRNA. In humans, Ago2 is loaded with miRNAs by the action of a specialized assembly called the RISC-loading complex (RLC), comprising the proteins Ago2, Dicer, and TRBP. Here we show that the human RLC assembles spontaneously in vitro from purified components. No cofactors or chaperones are required for the complex to form. T...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.0710869105
Journal:
Proceedings of the National Academy of Sciences of the United States of America More from this journal
Volume:
105
Issue:
2
Pages:
512-517
Publication date:
2008-01-01
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Language:
English
Keywords:
Pubs id:
pubs:59411
UUID:
uuid:fc711f75-59e1-4de5-81d9-bb5d33a243d2
Local pid:
pubs:59411
Source identifiers:
59411
Deposit date:
2012-12-19

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