Journal article icon

Journal article

A high-content screening platform utilizing polarization anisotropy and FLIM microscopy

Abstract:

An automated high-content screening microscope has been developed which uses fluorescence anisotropy imaging and fluorescence lifetime microscopy to identify Förster resonant energy transfer between eGFP and mRPF1 in drug screening assays. A wide-field polarization resolved imager is used to simultaneously capture the parallel and perpendicular components of both eGFP and mRFP1 fluorescence emission to provide a high-speed measurement of acceptor depolarization. Donor excited state lifetime m...

Expand abstract

Actions


Access Document


Publisher copy:
10.1117/12.763310

Authors


Journal:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE More from this journal
Volume:
6859
Publication date:
2008-01-01
DOI:
ISSN:
1605-7422
Language:
English
Keywords:
Pubs id:
pubs:107229
UUID:
uuid:fc57c63b-3cb5-4330-90ce-ab0383ee5062
Local pid:
pubs:107229
Source identifiers:
107229
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP