Journal article icon

Journal article

Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes.

Abstract:

Using total-internal-reflection fluorescence microscopy equipped with alternating-laser excitation, we were able to detect abortive initiation and promoter escape within single immobilized transcription complexes. Our approach uses fluorescence resonance energy transfer to monitor distances between a fluorescent probe incorporated in RNA polymerase (RNAP) and a fluorescent probe incorporated in DNA. We observe small, but reproducible and abortive-product-length-dependent, decreases in distanc...

Expand abstract

Actions


Access Document


Publisher copy:
10.1529/biophysj.105.069252

Authors


Journal:
Biophysical journal More from this journal
Volume:
90
Issue:
4
Pages:
1419-1431
Publication date:
2006-02-01
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Language:
English
Keywords:
Pubs id:
pubs:29496
UUID:
uuid:fb92adf1-1358-4bde-9e81-ee6de2103557
Local pid:
pubs:29496
Source identifiers:
29496
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP