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Extrinsic protein stabilization by the naturally occurring osmolytes beta-hydroxyectoine and betaine.

Abstract:

Thermodynamic aspects of protein stabilization by two widespread naturally occurring osmolytes, beta-hydroxyectoine and betaine, were studied using differential scanning calorimetry (DSC) and bovine ribonuclease A (RNase A) as a model protein. The osmolyte beta-hydroxyectoine purified from Marinococcus was found to be a very efficient stabilizer. At a concentration of 3M it increased the melting temperature of RNase A (Tm) by more than 12K and gave rise to a stability increase of 10.6kJ/mol a...

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Publication status:
Published

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Publisher copy:
10.1007/s007920050116

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
Journal:
Extremophiles : life under extreme conditions More from this journal
Volume:
3
Issue:
3
Pages:
191-198
Publication date:
1999-08-01
DOI:
EISSN:
1433-4909
ISSN:
1431-0651
Language:
English
Keywords:
Pubs id:
pubs:375461
UUID:
uuid:fb5f0ce7-0e08-488e-a03e-4f13efbdc1cf
Local pid:
pubs:375461
Source identifiers:
375461
Deposit date:
2013-11-16

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