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Gauging the flexibility of fluorescent markers for the interpretation of fluorescence resonance energy transfer.

Abstract:

Intramolecular distances in proteins and other biomolecules can be studied in living cells by means of fluorescence resonance energy transfer (FRET) in steady-state or pulsed-excitation experiments. The major uncertainty originates from the unknown orientation between the optical dipole moments of the fluorescent markers, especially when the molecule undergoes thermal fluctuations in physiological conditions. We introduce a statistical method based on the von Mises-Fisher distribution for the...

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Publication status:
Published

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Publisher copy:
10.1021/ja105720j

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Role:
Author
Journal:
Journal of the American Chemical Society
Volume:
133
Issue:
2
Pages:
279-285
Publication date:
2011-01-05
DOI:
EISSN:
1520-5126
ISSN:
0002-7863
URN:
uuid:fb12867e-2d78-483c-bedf-2207feae0fd6
Source identifiers:
399993
Local pid:
pubs:399993

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