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An efficient purification system for native minichromosome from Saccharomyces cerevisiae.

Abstract:
We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.

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Publisher copy:
10.1007/978-1-61779-477-3_8

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.) More from this journal
Volume:
833
Pages:
115-123
Publication date:
2012-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Language:
English
Keywords:
Pubs id:
pubs:228692
UUID:
uuid:faedcf0c-6f98-4313-9a8b-4a0c67ffe852
Local pid:
pubs:228692
Source identifiers:
228692
Deposit date:
2012-12-19

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