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Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION

Abstract:

Electron cryo-tomography (cryo-ET) is a technique that is used to produce 3D pictures (tomograms) of complex objects such as asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-microscopy (cryo-EM) images. Averaging of macromolecular complexes found within tomograms is known as subtomogram averaging, and this technique allows structure determination of macromolecular complexes in situ. Subtomogram averaging is also gaining in popularity for the calcula...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1038/nprot.2016.124

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
ORCID:
0000-0002-0168-0277
More by this author
ORCID:
0000-0002-0462-6540
European Molecular Biology Organization More from this funder
Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Protocols Journal website
Volume:
11
Issue:
11
Pages:
2054-2065
Publication date:
2016-07-04
Acceptance date:
2016-07-04
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pubs id:
pubs:699413
URN:
uri:faaf5298-1ccd-493d-a808-c1bb687e1747
UUID:
uuid:faaf5298-1ccd-493d-a808-c1bb687e1747
Local pid:
pubs:699413

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