Journal article
Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION
- Abstract:
-
Electron cryo-tomography (cryo-ET) is a technique that is used to produce 3D pictures (tomograms) of complex objects such as asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-microscopy (cryo-EM) images. Averaging of macromolecular complexes found within tomograms is known as subtomogram averaging, and this technique allows structure determination of macromolecular complexes in situ. Subtomogram averaging is also gaining in popularity for the calcula...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Accepted manuscript, pdf, 240.8KB)
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- Publisher copy:
- 10.1038/nprot.2016.124
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Authors
Funding
European Molecular Biology Organization
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Bibliographic Details
- Publisher:
- Nature Publishing Group Publisher's website
- Journal:
- Nature Protocols Journal website
- Volume:
- 11
- Issue:
- 11
- Pages:
- 2054-2065
- Publication date:
- 2016-07-04
- Acceptance date:
- 2016-07-04
- DOI:
- EISSN:
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1750-2799
- ISSN:
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1754-2189
- Pmid:
-
27685097
- Source identifiers:
-
699413
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:699413
- UUID:
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uuid:faaf5298-1ccd-493d-a808-c1bb687e1747
- Local pid:
- pubs:699413
- Deposit date:
- 2018-04-11
Terms of use
- Copyright holder:
- Nature America
- Copyright date:
- 2016
- Notes:
- © 2016 Nature America, Inc. All rights reserved. This is the accepted manuscript version of the article. The final version is available online from the Nature Publishing Group at: http://dx.doi.org/10.1038/nprot.2016.124
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