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Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION

Abstract:

Electron cryo-tomography (cryo-ET) is a technique that is used to produce 3D pictures (tomograms) of complex objects such as asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-microscopy (cryo-EM) images. Averaging of macromolecular complexes found within tomograms is known as subtomogram averaging, and this technique allows structure determination of macromolecular complexes in situ. Subtomogram averaging is also gaining in popularity for the calcula...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nprot.2016.124

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0002-0168-0277
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Role:
Author
ORCID:
0000-0002-0462-6540
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Name:
Medical Research Council
Grant:
MC_UP_A025_1013
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Name:
European Molecular Biology Organization
Publisher:
Nature Publishing Group
Journal:
Nature Protocols More from this journal
Volume:
11
Issue:
11
Pages:
2054-2065
Publication date:
2016-07-04
Acceptance date:
2016-07-04
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pmid:
27685097
Language:
English
Keywords:
Pubs id:
pubs:699413
UUID:
uuid:faaf5298-1ccd-493d-a808-c1bb687e1747
Local pid:
pubs:699413
Source identifiers:
699413
Deposit date:
2018-04-11

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