- Abstract:
-
Electron cryo-tomography (cryo-ET) is a technique that is used to produce 3D pictures (tomograms) of complex objects such as asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-microscopy (cryo-EM) images. Averaging of macromolecular complexes found within tomograms is known as subtomogram averaging, and this technique allows structure determination of macromolecular complexes in situ. Subtomogram averaging is also gaining in popularity for the calcula...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Accepted manuscript
- Files:
-
-
(pdf, 240.8kb)
-
- Publisher copy:
- 10.1038/nprot.2016.124
-
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- Publisher:
- Nature Publishing Group Publisher's website
- Journal:
- Nature Protocols Journal website
- Volume:
- 11
- Issue:
- 11
- Pages:
- 2054-2065
- Publication date:
- 2016-07-04
- Acceptance date:
- 2016-07-04
- DOI:
- EISSN:
-
1750-2799
- ISSN:
-
1754-2189
- Pubs id:
-
pubs:699413
- URN:
-
uri:faaf5298-1ccd-493d-a808-c1bb687e1747
- UUID:
-
uuid:faaf5298-1ccd-493d-a808-c1bb687e1747
- Local pid:
- pubs:699413
- Copyright holder:
- Nature America
- Copyright date:
- 2016
- Notes:
- © 2016 Nature America, Inc. All rights reserved. This is the accepted manuscript version of the article. The final version is available online from the Nature Publishing Group at: http://dx.doi.org/10.1038/nprot.2016.124
Journal article
Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION
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European Molecular Biology Organization
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