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Zooming-in on the proteome: very narrow-range immobilised pH gradients reveal more protein species and isoforms.

Abstract:

Two-dimensional gel electrophoresis (2-DE) enables separation of complex mixtures of proteins on a single polyacrylamide gel according to isoelectric point, molecular weight, solubility, and relative abundance. For this reason, 2-DE together with mass spectrometry (MS) has become a key technology in proteome analysis. The introduction of immobilised pH gradients (IPGs) for isoelectric focusing of proteins affords improved reproducibility and permits full-scale proteome analyses to be undertak...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
Journal:
Electrophoresis More from this journal
Volume:
22
Issue:
14
Pages:
2865-2871
Publication date:
2001-08-01
DOI:
EISSN:
1522-2683
ISSN:
0173-0835

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