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Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia

Abstract:

Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allow an earlier administration of a more appropriate antibiotic and could improve the outcome of these patients. The aim of this study was to develop a rapid protocol to identify the main microorganisms involved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples. First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL), endotrac...

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Publication status:
Published
Peer review status:
Peer reviewed

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Role:
Author
ORCID:
0000-0002-5046-4490
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Role:
Author
ORCID:
0000-0001-9454-062X
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Name:
Fundació Catalunya-La Pedrera
Grant:
PEP:HB-16-JF-VG-C
Publisher:
MDPI
Journal:
Microorganisms More from this journal
Volume:
8
Issue:
1
Article number:
103
Place of publication:
Switzerland
Publication date:
2020-01-11
Acceptance date:
2020-01-08
DOI:
EISSN:
2076-2607
Pmid:
31940771
Language:
English
Keywords:
Pubs id:
1083788
Local pid:
pubs:1083788
Deposit date:
2020-04-06

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