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Simultaneous visualization of FISH signals and bromo-deoxyuridine incorporation by formamide-free DNA denaturation.

Abstract:

The replication timing of different DNA sequences in the mammalian cell nucleus is a tightly regulated system, which affects important cellular processes such as genes expression, chromatin epigenetic marking, and maintenance of chromosome structure. For this reason, it is important to study the replication properties of specific sequences, to determine for example, if the replication timing varies in different tissues, or in the presence of specific reagents, such as hormones, or other biolo...

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Journal:
Methods in molecular biology (Clifton, N.J.) More from this journal
Volume:
659
Pages:
203-218
Publication date:
2010-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Language:
English
Keywords:
Pubs id:
pubs:71147
UUID:
uuid:f9dc027f-64cc-4889-8d44-deb757418235
Local pid:
pubs:71147
Source identifiers:
71147
Deposit date:
2013-02-20

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