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Going soft and SAD with manganese.

Abstract:

SAD phasing has been revisited recently, with experiments being carried out using previously unconventional sources of anomalous signal, particularly lighter atoms and softer X-rays. A case study is reported using the 75 kDa RNA-dependent RNA polymerase of the bacteriophase phi6, which binds a Mn atom and crystallizes with three molecules in the asymmetric unit. X-ray diffraction data were collected at a wavelength of 1.89 A and although the calculated anomalous signal from the three Mn atoms...

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Publication status:
Published

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Publisher copy:
10.1107/s0907444904026800

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Acta crystallographica. Section D, Biological crystallography More from this journal
Volume:
61
Issue:
Pt 1
Pages:
108-111
Publication date:
2005-01-01
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
Language:
English
Keywords:
Pubs id:
pubs:21154
UUID:
uuid:f9766e98-ba37-4879-a565-7b03758475f0
Local pid:
pubs:21154
Source identifiers:
21154
Deposit date:
2012-12-19

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