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Journal article

Quantitative high-throughput analysis of transcription factor binding specificities.

Abstract:

We present a general high-throughput approach to accurately quantify DNA-protein interactions, which can facilitate the identification of functional genetic polymorphisms. The method tested here on two structurally distinct transcription factors (TFs), NF-kappaB and OCT-1, comprises three steps: (i) optimized selection of DNA variants to be tested experimentally, which we show is superior to selecting variants at random; (ii) a quantitative protein-DNA binding assay using microarray and surfa...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gnh042

Authors


Linnell, J More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, WTC Human Genetics
Ragoussis, J More by this author
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Journal:
Nucleic acids research
Volume:
32
Issue:
4
Pages:
e44
Publication date:
2004
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:f9723e4f-882a-41b6-9834-7595d465f9a3
Source identifiers:
32638
Local pid:
pubs:32638

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