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Happy mapping: linkage mapping using a physical analogue of meiosis.

Abstract:

We have devised a simple method for ordering markers on a chromosome and determining the distances between them. It uses haploid equivalents of DNA and the polymerase chain reaction, hence 'happy mapping'. Our approach is analogous to classical linkage mapping; we replace its two essential elements, chromosome breakage and segregation, by in vitro analogues. DNA from any source is broken randomly by gamma-irradiation or shearing. Markers are then segregated by diluting the resulting fragments...

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Publication status:
Published

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Publisher copy:
10.1093/nar/21.1.13

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Nucleic acids research
Volume:
21
Issue:
1
Pages:
13-20
Publication date:
1993-01-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Source identifiers:
28757
Language:
English
Keywords:
Pubs id:
pubs:28757
UUID:
uuid:f925d0d7-e4fa-4683-90ee-2fb7b37cda61
Local pid:
pubs:28757
Deposit date:
2012-12-19

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