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Specific transcriptional pausing activates polyadenylation in a coupled in vitro system.

Abstract:

We have developed a coupled in vitro transcription-polyadenylation system to investigate RNA polymerase II (Pol II) termination, which depends on active polyadenylation of the nascent RNA. Specific G-rich sequences originally identified as binding sites for the transcription factor MAZ both pause Pol II and activate polyadenylation of an upstream poly(A) signal. They do not affect polyadenylation efficiency in an uncoupled cleavage assay. In contrast, pausing of Pol II elongation induced by a...

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Publication status:
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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Molecular cell More from this journal
Volume:
3
Issue:
5
Pages:
593-600
Publication date:
1999-05-01
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Language:
English
Keywords:
Pubs id:
pubs:19601
UUID:
uuid:f912e40b-d754-4089-9124-9af9f98a1d45
Local pid:
pubs:19601
Source identifiers:
19601
Deposit date:
2012-12-19

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