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A miniaturized screen for inhibitors of Jumonji histone demethylases.

Abstract:

2-Oxoglutarate- and Fe(ii)-dependent oxygenases are a major class of N(epsilon)-methyl lysine demethylases that are involved in epigenetic regulation. Assays suitable for implementation in a high-throughput manner have been lacking for these enzymes. Here, we describe the design and implementation of a robust and miniaturized high-throughput kinetic assay for inhibitors of JMJD2E using a formaldehyde dehydrogenase-coupled reaction with real-time fluorescence detection. Reactant compatibility ...

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Journal:
Molecular BioSystems
Volume:
6
Issue:
2
Pages:
357-364
Publication date:
2010-01-01
ISSN:
1742-2051
URN:
uuid:f8f61f20-8ae3-46a6-b618-3c10a9472c9d
Local pid:
SGC:20094655

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