Journal article icon

Journal article

Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism.

Abstract:

Using fluorescence resonance energy transfer to monitor distances within single molecules of abortively initiating transcription initiation complexes, we show that initial transcription proceeds through a "scrunching" mechanism, in which RNA polymerase (RNAP) remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center. We show further that putative alternative mechanisms for RNAP active-center translocation in initial transcription, involving "transient excur...

Expand abstract

Actions


Access Document


Publisher copy:
10.1126/science.1131399

Authors


Journal:
Science (New York, N.Y.) More from this journal
Volume:
314
Issue:
5802
Pages:
1144-1147
Publication date:
2006-11-01
DOI:
EISSN:
1095-9203
ISSN:
0036-8075
Language:
English
Keywords:
Pubs id:
pubs:21275
UUID:
uuid:f8cace15-b23a-4168-939e-5a5fc0371f4a
Local pid:
pubs:21275
Source identifiers:
21275
Deposit date:
2012-12-20

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP