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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

Abstract:

Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here, to address this limitation, we present a screening platform which allows simultaneous measurements of the promoter strength, 5', and 3' processing efficiencies across a library of tRNA variants. This analysis reveals that the sequence determinants underlying these ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-019-09148-3

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Clinical Laboratory Sciences
Role:
Author
ORCID:
0000-0002-2161-9959
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Role:
Author
ORCID:
0000-0002-6857-5922
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Name:
Biotechnology and Biological Sciences Research Council
Grant:
BB/N006550/1
Publisher:
Springer Nature
Journal:
Nature Communications More from this journal
Volume:
10
Article number:
1490
Place of publication:
England
Publication date:
2019-04-02
Acceptance date:
2019-02-22
DOI:
EISSN:
2041-1723
Pmid:
30940799

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