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A simple method for the evaluation of receptor binding capacity of modified cytokines.

Abstract:

We have developed a flow cytometric method to evaluate the binding of interleukin-2 analogues to receptors. The method relies on competition for binding between a fluorescein-conjugated monoclonal antibody (MoAb) directed against the human interleukin-2 receptor alpha chain (fluorescein isothiocyanate (FITC) anti-IL-2R) and the test protein. IL-2R positive cells are incubated with FITC-anti-IL-2R MoAb in the presence of native IL-2 or IL-2 iodinated by either the chloramine-T or the lactopero...

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Journal:
Journal of immunological methods More from this journal
Volume:
166
Issue:
2
Pages:
177-182
Publication date:
1993-12-01
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
Language:
English
Keywords:
Pubs id:
pubs:35828
UUID:
uuid:f5f59685-d2ac-4a9c-8cee-8f3ab1f2d590
Local pid:
pubs:35828
Source identifiers:
35828
Deposit date:
2012-12-20

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