Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.

Journal article

OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. DESIGN AND METHODS: PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be i...

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Authors: Végvári, Á; Rezeli, M; Sihlbom, C; Häkkinen, J; Carlsohn, E

• Journal: Clinical biochemistry
• Volume: 45
• Issue: 4-5
• Pages: 331-338
• Publication date: 2012-03-01
• DOI: 10.1016/j.clinbiochem.2011.11.018
• EISSN: 1873-2933
• ISSN: 0009-9120
• Language: English
• Keywords: Infertility, Male; Blotting, Western; Tandem Mass Spectrometry; Electrophoresis, Polyacrylamide Gel; Peptide Mapping; Prostate-Specific Antigen; Semen; Reproducibility of Results; Proteolysis; Proteomics; Middle Aged; Adult; Male; Biological Markers; Spectrometry, Mass, Electrospray Ionization; Humans; Cluster Analysis; Isoenzymes; Molecular Weight; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization